Longitudinal inhibition of PI3K/Akt/mTOR signaling by LY294002 and rapamycin induces growth arrest of adult T-cell leukemia cells

T Ikezoe, C Nishioka, K Bandobashi, Y Yang… - Leukemia research, 2007 - Elsevier
This study found that phosphatidylinositol 3-kinase (PI3K)/Akt/mammalian target of
rapamycin (mTOR) signaling was activated in human T-cell lymphotropic virus type I (HTLV-
1)-infected leukemia cells. Rapamycin (1–100nM, 48h), the inhibitor of mTOR and its analog
RAD001 (1–100nM, 48h)-induced growth inhibition and G0/G1 cell cycle arrest of these
cells in association with de-phosphorylation of p70S6K and 4E-BP-1, although IC50 was not
achieved. Paradoxically, rapamycin-stimulated phosphorylation of Akt at Ser473. Blockade …