[PDF][PDF] Serotonin increases calcium current in human atrial myocytes via the newly described 5-hydroxytryptamine4 receptors.
H Ouadid, J Seguin, A Dumuis, J Bockaert… - Molecular …, 1992 - Citeseer
H Ouadid, J Seguin, A Dumuis, J Bockaert, J Nargeot
Molecular pharmacology, 1992•CiteseerIn various species, including humans, 5-hydroxytryptamine(5-HI) has been shown to exert
positive chronotropic and inotropic cardiac effects through different types of receptors. The
goal of the present study was to investigate the regulation by 5-HT of voltage-gated Ca2
channels in human atrial myocytes and to characterize the receptor involved.
Cardiomyocytes isolated en-zymatically and mechanically were voltage-clamped using the
whole-cell configuration of the patch-clamp technique. Extracellular perfusion of 5-HT …
positive chronotropic and inotropic cardiac effects through different types of receptors. The
goal of the present study was to investigate the regulation by 5-HT of voltage-gated Ca2
channels in human atrial myocytes and to characterize the receptor involved.
Cardiomyocytes isolated en-zymatically and mechanically were voltage-clamped using the
whole-cell configuration of the patch-clamp technique. Extracellular perfusion of 5-HT …
Summary
In various species, including humans, 5-hydroxytryptamine(5-HI) has been shown to exert positive chronotropic and inotropic cardiac effects through different types of receptors. The goal of the present study was to investigate the regulation by 5-HT of voltage-gated Ca2 channels in human atrial myocytes and to characterize the receptor involved. Cardiomyocytes isolated en-zymatically and mechanically were voltage-clamped using the whole-cell configuration of the patch-clamp technique. Extracellular perfusion of 5-HT increased Ca2 current(Ica) amplitude with a EC50 (0.1 tM) similar to that observed with isoprenaline. The effects of 5-HT were blocked by the addition of protein kinase A inhibitor in the pipette. In addition, the effects of 5-HT, isoprenaline, and intracellular cAMP on lCa were not additive.
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