Methods for the analysis of centrosome reproduction in cancer cells

WL Lingle, JL Salisbury - Methods in cell biology, 2001 - Elsevier
Publisher Summary This chapter discusses methods for the analysis of centrosome
reproduction in cancer cells. The chapter describes procedures and considerations for—
namely,(1) tissue procurement and storage,(2) Xenopus egg extract preparation and
storage,(3) reaction mixture composition and application, and (4) experimental design and
data analysis. The assay described in this chapter allows a direct comparison of centrosome
function between normal and tumor tissues. It can be applied to samples such as human …