Serum-induced proliferation of rabbit aortic smooth muscle cells from the contractile state is inhibited by 8-Br-cAMP but not 8-Br-cGMP
K Southgate, AC Newby - Atherosclerosis, 1990 - Elsevier
A method is described for the quantification of vascular smooth muscle cell growth from
individual explants of contractile rabbit aortic tunica media. The precision of the method
probably depends on regular explant geometry (1-mm squares) and pooling sufficient
explants. Serum-induced growth was quantified by measurements of ATP concentration,
incorporation of [3 H] thymidine and DNA concentration. The possible effects of endogenous
vasodilator agents on growth were investigated by using lipid soluble analogues of their …
individual explants of contractile rabbit aortic tunica media. The precision of the method
probably depends on regular explant geometry (1-mm squares) and pooling sufficient
explants. Serum-induced growth was quantified by measurements of ATP concentration,
incorporation of [3 H] thymidine and DNA concentration. The possible effects of endogenous
vasodilator agents on growth were investigated by using lipid soluble analogues of their …