[HTML][HTML] Calpain activity regulates the cell surface distribution of amyloid precursor protein: inhibition of calpains enhances endosomal generation of β-cleaved C …
PM Mathews, Y Jiang, SD Schmidt, OM Grbovic… - Journal of Biological …, 2002 - ASBMB
In murine L cells, treatment with calpeptin or calpain inhibitor III increased Aβ42, but not
Aβ40, secretion in a dose-dependent fashion. This correlated with an increase in the levels
of amyloid precursor protein (APP) carboxyl-terminal fragments (CTFs). Immunoprecipitation
with novel mAbs directed against the carboxyl-terminus of APP or specific for the β-cleaved
CTF showed that generation of both α-and β-cleaved CTFs increase proportionately
following inhibition of calpains. Pulse-chase metabolic labeling confirmed that inhibiting …
Aβ40, secretion in a dose-dependent fashion. This correlated with an increase in the levels
of amyloid precursor protein (APP) carboxyl-terminal fragments (CTFs). Immunoprecipitation
with novel mAbs directed against the carboxyl-terminus of APP or specific for the β-cleaved
CTF showed that generation of both α-and β-cleaved CTFs increase proportionately
following inhibition of calpains. Pulse-chase metabolic labeling confirmed that inhibiting …