Phage display of cDNA repertoires: the pVI display system and its applications for the selection of immunogenic ligands
SE Hufton, PT Moerkerk, EV Meulemans… - Journal of …, 1999 - Elsevier
The selection of phage displayed cDNA repertoires on an immobilised target has been
reported to be an efficient way to rapidly identify interacting partners. To date, however, only
a few successful applications have been reported. Here, we present a review of the current
status of the display and selection of cDNA libraries using phage. As an example, we report
the construction of a set of phage display vectors suitable for cDNA display based on fusion
to the minor bacteriophage coat protein 6 (pVI) of filamentous phage. We have evaluated …
reported to be an efficient way to rapidly identify interacting partners. To date, however, only
a few successful applications have been reported. Here, we present a review of the current
status of the display and selection of cDNA libraries using phage. As an example, we report
the construction of a set of phage display vectors suitable for cDNA display based on fusion
to the minor bacteriophage coat protein 6 (pVI) of filamentous phage. We have evaluated …