[HTML][HTML] Isolation and biochemical characterization of the human Dkk-1 homologue, a novel inhibitor of mammalian Wnt signaling
P Fedi, A Bafico, AN Soria, WH Burgess, T Miki… - Journal of biological …, 1999 - ASBMB
In an effort to isolate novel growth factors, we identified a human protein, designated Sk, that
co-eluted with Neuregulin during chromatographic separation of conditioned medium from
the SK-LMS-1 human leiomyosarcoma cell line. Degenerate oligonucleotides based on
amino-terminal sequence analysis of the purified protein were used to isolate the
corresponding cDNA from a library generated from this cell line. Sk is a novel 266-amino
acid protein that contains a signal peptide sequence and two cysteine-rich domains with no …
co-eluted with Neuregulin during chromatographic separation of conditioned medium from
the SK-LMS-1 human leiomyosarcoma cell line. Degenerate oligonucleotides based on
amino-terminal sequence analysis of the purified protein were used to isolate the
corresponding cDNA from a library generated from this cell line. Sk is a novel 266-amino
acid protein that contains a signal peptide sequence and two cysteine-rich domains with no …