Variability in the measurement of hERG potassium channel inhibition: effects of temperature and stimulus pattern
GE Kirsch, ES Trepakova, JC Brimecombe… - … of pharmacological and …, 2004 - Elsevier
INTRODUCTION: In vitro evaluation of drug effects on hERG K+ channels is a valuable tool
for identifying potential proarrhythmic side effects in drug safety testing. Patch-clamp
recording of hERG K+ current in mammalian cells can accurately evaluate drug effects, but
the methodology has not been standardized, and results vary widely. Our objective was to
evaluate two potential sources of variability: the temperature at which recordings are
performed and the voltage pulse protocol used to activate hERG K+ channels expressed in …
for identifying potential proarrhythmic side effects in drug safety testing. Patch-clamp
recording of hERG K+ current in mammalian cells can accurately evaluate drug effects, but
the methodology has not been standardized, and results vary widely. Our objective was to
evaluate two potential sources of variability: the temperature at which recordings are
performed and the voltage pulse protocol used to activate hERG K+ channels expressed in …