Potential role of lipid peroxidation derived DNA damage in human colon carcinogenesis: studies on exocyclic base adducts as stable oxidative stress markers
H Bartsch, J Nair - Cancer Detection and Prevention, 2002 - Elsevier
Molecular pathways to colorectal cancer involve multiple genetic changes that may be
caused by overproduction of reactive oxygen species in cancer-related genes. Our aim was
to investigate, whether besides direct oxidative DNA damage, reactive oxygen and nitrogen
species induce lipid peroxidation (LPO) that could yield etheno-DNA adducts via trans-4-
hydroxy-2-nonenal, a major aldehyde generated by LPO, in colon tissue. We analyzed the
etheno-DNA adducts by a highly specific, ultrasensitive method involving immunoaffinity …
caused by overproduction of reactive oxygen species in cancer-related genes. Our aim was
to investigate, whether besides direct oxidative DNA damage, reactive oxygen and nitrogen
species induce lipid peroxidation (LPO) that could yield etheno-DNA adducts via trans-4-
hydroxy-2-nonenal, a major aldehyde generated by LPO, in colon tissue. We analyzed the
etheno-DNA adducts by a highly specific, ultrasensitive method involving immunoaffinity …