Allelic discrimination using fluorogenic probes and the 5′ nuclease assay
KJ Livak - Genetic analysis: biomolecular engineering, 1999 - Elsevier
Large-scale screening for known polymorphisms will require techniques with few steps and
the ability to automate each of these steps. In this regard, the 5′ nuclease, or TaqMan, PCR
assay is especially attractive. A fluorogenic probe, consisting of an oligonucleotide labeled
with both a fluorescent reporter dye and a quencher dye, is included in a typical PCR.
Amplification of the probe-specific product causes cleavage of the probe, generating an
increase in reporter fluorescence. By using different reporter dyes, cleavage of allele …
the ability to automate each of these steps. In this regard, the 5′ nuclease, or TaqMan, PCR
assay is especially attractive. A fluorogenic probe, consisting of an oligonucleotide labeled
with both a fluorescent reporter dye and a quencher dye, is included in a typical PCR.
Amplification of the probe-specific product causes cleavage of the probe, generating an
increase in reporter fluorescence. By using different reporter dyes, cleavage of allele …