Mechanisms involved in Helicobacter pylori-induced interleukin-8 production by a gastric cancer cell line, MKN45

M Aihara, D Tsuchimoto, H Takizawa… - Infection and …, 1997 - Am Soc Microbiol
M Aihara, D Tsuchimoto, H Takizawa, A Azuma, H Wakebe, Y Ohmoto, K Imagawa…
Infection and immunity, 1997Am Soc Microbiol
Accumulating evidence suggests an important role of interleukin-8 (IL-8) in Helicobacter
pylori infection-associated chronic atrophic gastritis and peptic ulcer. We observed in this
study that a gastric cancer-derived cell line, MKN45, produced a massive amount of IL-8
upon coculture with live H. pylori but not with killed H. pylori, H. pylori culture supernatants,
or live H. pylori separated by a permeable membrane, indicating that IL-8 production
requires a direct contact between the cells and live bacteria. Moreover, the tyrosine kinase …
Accumulating evidence suggests an important role of interleukin-8 (IL-8) in Helicobacter pylori infection-associated chronic atrophic gastritis and peptic ulcer. We observed in this study that a gastric cancer-derived cell line, MKN45, produced a massive amount of IL-8 upon coculture with live H. pylori but not with killed H. pylori, H. pylori culture supernatants, or live H. pylori separated by a permeable membrane, indicating that IL-8 production requires a direct contact between the cells and live bacteria. Moreover, the tyrosine kinase inhibitor herbimycin but neither a protein kinase C inhibitor (staurosporine) nor a protein kinase A inhibitor (H89) inhibited IL-8 production by MKN45 cells cocultured with live bacteria, suggesting the involvement of a tyrosine kinase(s) in H. pylori-induced IL-8 production. In addition, coculture of H. pylori induced IL-8 mRNA expression in MKN45 cells and an increase in luciferase activity in cells which were transfected with a luciferase expression vector linked with a 5'-flanking region of the IL-8 gene (bp -133 to +44), indicating that the induction of IL-8 production occurred at the transcriptional level. This region contain three cis elements important for induction of IL-8 gene expression: AP-1 (-126 to -120 bp), NF-IL6 (-94 to -81 bp), and NF-kappaB (-80 to -70 bp) binding sites. Mutation of the NF-kappaB binding site abrogated completely the induction of luciferase activity, whereas that of the AP-1 site partially reduced the induction. However, mutation of the NF-IL6 binding site resulted in no decrease in the induction of luciferase activity. Moreover, specific NF-kappaB complexes were detected in the nuclear proteins extracted from MKN45 cells which were infected with H. pylori. Collectively, these results suggest that H. pylori induced the activation of NF-kappaB as well as AP-1, leading to IL-8 gene transcription.
American Society for Microbiology