[HTML][HTML] Roles of catalytic domain residues in interfacial binding and activation of group IV cytosolic phospholipase A2
Group IV cytosolic phospholipase A 2 (cPLA 2) has been shown to play a critical role in
eicosanoid biosynthesis. cPLA 2 is composed of the C2 domain that mediates the Ca 2+-
dependent interfacial binding of protein and the catalytic domain. To elucidate the
mechanism of interfacial activation of cPLA 2, we measured the effects of mutations of
selected ionic and hydrophobic residues in the catalytic domain on the enzyme activity and
the membrane binding of cPLA 2. Mutations of anionic residues located on (Glu 419 and Glu …
eicosanoid biosynthesis. cPLA 2 is composed of the C2 domain that mediates the Ca 2+-
dependent interfacial binding of protein and the catalytic domain. To elucidate the
mechanism of interfacial activation of cPLA 2, we measured the effects of mutations of
selected ionic and hydrophobic residues in the catalytic domain on the enzyme activity and
the membrane binding of cPLA 2. Mutations of anionic residues located on (Glu 419 and Glu …