Transduction of Schistosoma mansoni by vesicular stomatitis virus glycoprotein-pseudotyped Moloney murine leukemia retrovirus
KJ Kines, VH Mann, ME Morales, BD Shelby… - Experimental …, 2006 - Elsevier
Retroviral transduction of cultured schistosomes offers a potential means to establish
transgenic lines of schistosomes and thereby to facilitate the elucidation of schistosome
gene function and expression. The Moloney murine leukemia retroviral (MMLV) vector
pLNHX was modified to incorporate EGFP or luciferase reporter genes under control of
schistosome endogenous gene promoters from the spliced leader RNA and HSP70 genes.
These constructs and a plasmid encoding vesicular stomatitis virus glycoprotein (VSVG) …
transgenic lines of schistosomes and thereby to facilitate the elucidation of schistosome
gene function and expression. The Moloney murine leukemia retroviral (MMLV) vector
pLNHX was modified to incorporate EGFP or luciferase reporter genes under control of
schistosome endogenous gene promoters from the spliced leader RNA and HSP70 genes.
These constructs and a plasmid encoding vesicular stomatitis virus glycoprotein (VSVG) …