Real-time reverse transcription PCR detection of norovirus, sapovirus and astrovirus as causative agents of acute viral gastroenteritis
C Logan, JJ O'Leary, N O'Sullivan - Journal of virological methods, 2007 - Elsevier
The design and development of highly sensitive real-time reverse transcription PCR assays
for the detection of norovirus genogroups I, II and IV, sapovirus genogroups I, II and IV, and
human astrovirus from stool samples is described. Examination of 140 stool samples from
paediatric patients exhibiting symptoms of diarrhoea and/or vomiting resulted in increased
detection levels as compared to examination by electron microscopy. Real-time PCR
resulted in a 200% increase in the rate of detection of norovirus as compared to electron …
for the detection of norovirus genogroups I, II and IV, sapovirus genogroups I, II and IV, and
human astrovirus from stool samples is described. Examination of 140 stool samples from
paediatric patients exhibiting symptoms of diarrhoea and/or vomiting resulted in increased
detection levels as compared to examination by electron microscopy. Real-time PCR
resulted in a 200% increase in the rate of detection of norovirus as compared to electron …