A common polymorphism in the interleukin 8 gene promoter is associated with Clostridium difficile diarrhea
Official journal of the American College of Gastroenterology| ACG, 2006•journals.lww.com
OBJECTIVE Mucosal interleukin 8 (IL-8) and neutrophil recruitment are central to the
pathogenesis of Clostridium difficile (CD) toxin-induced diarrhea (CDD). We hypothesized
that like other inflammatory mucosal infections, susceptibility to CDD would relate to
genetically determined variations in the production of IL-8. METHODS Fecal IL-8 production
and single nucleotide polymorphism (SNP) frequency in the− 251 region of the IL-8 gene
were determined in hospitalized patients: 42 with CDD, 42 with CD-negative diarrhea, and …
pathogenesis of Clostridium difficile (CD) toxin-induced diarrhea (CDD). We hypothesized
that like other inflammatory mucosal infections, susceptibility to CDD would relate to
genetically determined variations in the production of IL-8. METHODS Fecal IL-8 production
and single nucleotide polymorphism (SNP) frequency in the− 251 region of the IL-8 gene
were determined in hospitalized patients: 42 with CDD, 42 with CD-negative diarrhea, and …
Abstract
OBJECTIVE
Mucosal interleukin 8 (IL-8) and neutrophil recruitment are central to the pathogenesis of Clostridium difficile (CD) toxin-induced diarrhea (CDD). We hypothesized that like other inflammatory mucosal infections, susceptibility to CDD would relate to genetically determined variations in the production of IL-8.
METHODS
Fecal IL-8 production and single nucleotide polymorphism (SNP) frequency in the− 251 region of the IL-8 gene were determined in hospitalized patients: 42 with CDD, 42 with CD-negative diarrhea, and 41 without diarrhea. Cases and controls were matched by age, length of hospital stay, comorbidity, and receipt of antibiotics.
RESULTS
An association was found between the IL-8− 251 A/A allele and occurrence of CDD, 39% versus 16%(OR= 3.26, 95% CI 1.09–9.17) and 17%(OR= 5.50, 95% CI 1.22–24.8) for the two control groups. Comparing results by IL-8 genotype for the CDD cases, median and mean fecal IL-8 levels were significantly higher for the− 251 A/A genotype (p= 0.03 for median and 0.001 for mean).
CONCLUSIONS
These studies indicate a common SNP in the IL-8 gene is associated with increased susceptibility to CDD and with increased fecal IL-8 in diarrheal stools.
Lippincott Williams & Wilkins