Participation of nitric oxide reductase in survival of Pseudomonas aeruginosa in LPS-activated macrophages
K Kakishima, A Shiratsuchi, A Taoka… - Biochemical and …, 2007 - Elsevier
K Kakishima, A Shiratsuchi, A Taoka, Y Nakanishi, Y Fukumori
Biochemical and biophysical research communications, 2007•ElsevierNitric oxide (NO) plays a crucial role in the antimicrobial activity of host defense systems. We
investigated the function of Pseudomonas aeruginosa NO reductase as a detoxifying
enzyme in phagocytes. We found that the growth of the NO reductase-deficient mutant of P.
aeruginosa under a microaerobic condition was inhibited by the exogenous NO.
Furthermore, the intracellular survival assay within the NO-producing RAW 264.7
macrophages revealed that the wild-type strain survived longer than the NO reductase …
investigated the function of Pseudomonas aeruginosa NO reductase as a detoxifying
enzyme in phagocytes. We found that the growth of the NO reductase-deficient mutant of P.
aeruginosa under a microaerobic condition was inhibited by the exogenous NO.
Furthermore, the intracellular survival assay within the NO-producing RAW 264.7
macrophages revealed that the wild-type strain survived longer than the NO reductase …
Nitric oxide (NO) plays a crucial role in the antimicrobial activity of host defense systems. We investigated the function of Pseudomonas aeruginosa NO reductase as a detoxifying enzyme in phagocytes. We found that the growth of the NO reductase-deficient mutant of P. aeruginosa under a microaerobic condition was inhibited by the exogenous NO. Furthermore, the intracellular survival assay within the NO-producing RAW 264.7 macrophages revealed that the wild-type strain survived longer than the NO reductase-deficient mutant. These results suggest that the P. aeruginosa NO reductase may contribute to the intracellular survival by acting as a counter component against the host’s defense systems.
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