Coordination between donor cell type and cell cycle stage improves nuclear cloning efficiency in cattle
DN Wells, G Laible, FC Tucker, AL Miller, JE Oliver… - Theriogenology, 2003 - Elsevier
Several studies have shown that both quiescent and proliferating somatic donor cells can be
fully reprogrammed after nuclear transfer (NT) and result in viable offspring. So far, however,
no comparative study has conclusively demonstrated the relative importance of donor cell
cycle stage on nuclear cloning efficiency. Here, we compare two different types of bovine
fetal fibroblasts (BFFs) that were synchronized in G0, G1, and different phases within G1. We
show that for non-transgenic (non-TG) fibroblasts, serum starvation into G0 results in a …
fully reprogrammed after nuclear transfer (NT) and result in viable offspring. So far, however,
no comparative study has conclusively demonstrated the relative importance of donor cell
cycle stage on nuclear cloning efficiency. Here, we compare two different types of bovine
fetal fibroblasts (BFFs) that were synchronized in G0, G1, and different phases within G1. We
show that for non-transgenic (non-TG) fibroblasts, serum starvation into G0 results in a …