Purification, characterization, and antitumor activity of nonrecombinant mouse tumor necrosis factor.
K Haranaka, EA Carswell… - Proceedings of the …, 1986 - National Acad Sciences
K Haranaka, EA Carswell, BD Williamson, JS Prendergast, N Satomi, LJ Old
Proceedings of the National Academy of Sciences, 1986•National Acad SciencesMouse tumor necrosis factor (TNF) was purified from serum through a series of steps, and
each step was monitored for L-cell cytotoxicity in vitro and tumor-necrotizing activity in vivo.
The two activities copurified and could not be dissociated. Purified mouse TNF has a specific
activity of 2.2 X 10 (7)(L-cell assay in the absence of actinomycin D) and 1 microgram
causes necrosis of the standard TNF-sensitive sarcoma Meth A. TNF has a Mr of 39,000+/-
2000 by gel filtration and a Mr of 16,000-18,000 by NaDodSO4/PAGE. Both molecular …
each step was monitored for L-cell cytotoxicity in vitro and tumor-necrotizing activity in vivo.
The two activities copurified and could not be dissociated. Purified mouse TNF has a specific
activity of 2.2 X 10 (7)(L-cell assay in the absence of actinomycin D) and 1 microgram
causes necrosis of the standard TNF-sensitive sarcoma Meth A. TNF has a Mr of 39,000+/-
2000 by gel filtration and a Mr of 16,000-18,000 by NaDodSO4/PAGE. Both molecular …
Mouse tumor necrosis factor (TNF) was purified from serum through a series of steps, and each step was monitored for L-cell cytotoxicity in vitro and tumor-necrotizing activity in vivo. The two activities copurified and could not be dissociated. Purified mouse TNF has a specific activity of 2.2 X 10(7) (L-cell assay in the absence of actinomycin D) and 1 microgram causes necrosis of the standard TNF-sensitive sarcoma Meth A. TNF has a Mr of 39,000 +/- 2000 by gel filtration and a Mr of 16,000-18,000 by NaDodSO4/PAGE. Both molecular weight forms display cytotoxic and necrotizing activities. TNF has a pI of 3.9 and is destroyed by trypsin, protease, elastase, and alpha-chymotrypsin but not by neuraminidase or papain. These characteristics of nonrecombinant mouse TNF clearly resemble those of recombinant human and mouse TNF.
National Acad Sciences