Autoantigen arrays for multiplex analysis of antibody isotypes
KL Graham, M Vaysberg, A Kuo, PJ Utz - Proteomics, 2006 - Wiley Online Library
KL Graham, M Vaysberg, A Kuo, PJ Utz
Proteomics, 2006•Wiley Online LibraryWe describe here a microarray‐based method for multiplexed, antigen‐specific assessment
of immunoglobulin (Ig) subclasses. We used 1152‐feature arrays composed of 140 antigens
or antigen fragments to detect isotype‐specific mAb, to quantitatively monitor changes in
isotype mAb concentration, and to profile antigen‐specific antibody isotype production in a
murine model of autoimmunity. This platform can be easily adapted to a variety of
applications, and has the potential to elucidate mechanisms that govern development and …
of immunoglobulin (Ig) subclasses. We used 1152‐feature arrays composed of 140 antigens
or antigen fragments to detect isotype‐specific mAb, to quantitatively monitor changes in
isotype mAb concentration, and to profile antigen‐specific antibody isotype production in a
murine model of autoimmunity. This platform can be easily adapted to a variety of
applications, and has the potential to elucidate mechanisms that govern development and …
Abstract
We describe here a microarray‐based method for multiplexed, antigen‐specific assessment of immunoglobulin (Ig) subclasses. We used 1152‐feature arrays composed of 140 antigens or antigen fragments to detect isotype‐specific mAb, to quantitatively monitor changes in isotype mAb concentration, and to profile antigen‐specific antibody isotype production in a murine model of autoimmunity. This platform can be easily adapted to a variety of applications, and has the potential to elucidate mechanisms that govern development and evolution of antibody responses in in vivo and in vitro systems.
Wiley Online Library