Developmental changes of aldehyde dehydrogenase isozymes in human livers: Mitochondrial ALDH2 isozyme is expressed in fetal livers
A Yoshida, A Shibuya, V Dave, M Nakayama… - Experientia, 1990 - Springer
A Yoshida, A Shibuya, V Dave, M Nakayama, A Hayashi
Experientia, 1990•SpringerPrevious reports suggested that the major cytosolic aldehyde dehydrogenase (ALDH 1) was
present in fetal and infant livers, but the major mitochondrial isozyme (ALDH 2) was absent
or severely diminished. Re-examination by means of starch gel electrophoresis followed by
enzyme activity staining, and by means of dot blot immuno-hybridization of liver samples
with known genotypes of the ALDH 2 locus, indicated that both ALDH 1 and ALDH 2 genes
are expressed in fetal and infant livers. In addition, ALDH 4 isozyme was also observed. The …
present in fetal and infant livers, but the major mitochondrial isozyme (ALDH 2) was absent
or severely diminished. Re-examination by means of starch gel electrophoresis followed by
enzyme activity staining, and by means of dot blot immuno-hybridization of liver samples
with known genotypes of the ALDH 2 locus, indicated that both ALDH 1 and ALDH 2 genes
are expressed in fetal and infant livers. In addition, ALDH 4 isozyme was also observed. The …
Summary
Previous reports suggested that the major cytosolic aldehyde dehydrogenase (ALDH1) was present in fetal and infant livers, but the major mitochondrial isozyme (ALDH2) was absent or severely diminished. Re-examination by means of starch gel electrophoresis followed by enzyme activity staining, and by means of dot blot immuno-hybridization of liver samples with known genotypes of theALDH 2 locus, indicated that bothALDH 1 andALDH 2 genes are expressed in fetal and infant livers. In addition, ALDH4 isozyme was also observed. The results imply that a fetus with the ‘usual’ homozygousALDH 2 1 /ALDH 2 1 genotype, but not one with the atypicalALDH 2 1 /ALDH 2 2 orALDH 2 2 /ALDH 2 2 , is capable of detoxifying acetaldehyde transferred from the mother.
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