Role of transglutaminase in [3H] 5-HT release from synaptosomes and in the inhibitory effect of tetanus toxin
M Gobbi, E Frittoli, T Mennini - Neurochemistry international, 1996 - Elsevier
It has been suggested that the Ca2+-dependent enzyme transglutaminase (TGase) may
suppress vesicular neurotransmitter release and mediate the inhibitory effect of tetanus toxin
(TetTx) on exocytosis. The aim of the present study was to test this in a model of [3H] 5-HT
release from superfused rat cortical synaptosomes. Monodansylcadaverine, a synthetic
amine that acts as an alternative substrate for TGase, showed dose-dependent releasing
activity which, however, was Ca2+-independent, being maintained in a Ca2+-free buffer …
suppress vesicular neurotransmitter release and mediate the inhibitory effect of tetanus toxin
(TetTx) on exocytosis. The aim of the present study was to test this in a model of [3H] 5-HT
release from superfused rat cortical synaptosomes. Monodansylcadaverine, a synthetic
amine that acts as an alternative substrate for TGase, showed dose-dependent releasing
activity which, however, was Ca2+-independent, being maintained in a Ca2+-free buffer …