Role of β2-integrins for homing and neovascularization capacity of endothelial progenitor cells

E Chavakis, A Aicher, C Heeschen, K Sasaki… - The Journal of …, 2005 - rupress.org
E Chavakis, A Aicher, C Heeschen, K Sasaki, R Kaiser, N El Makhfi, C Urbich, T Peters…
The Journal of experimental medicine, 2005rupress.org
The mechanisms of homing of endothelial progenitor cells (EPCs) to sites of ischemia are
unclear. Here, we demonstrate that ex vivo–expanded EPCs as well as murine
hematopoietic Sca-1+/Lin− progenitor cells express β2-integrins, which mediate the
adhesion of EPCs to endothelial cell monolayers and their chemokine-induced
transendothelial migration in vitro. In a murine model of hind limb ischemia, Sca-1+/Lin−
hematopoietic progenitor cells from β2-integrin–deficient mice are less capable of homing to …
The mechanisms of homing of endothelial progenitor cells (EPCs) to sites of ischemia are unclear. Here, we demonstrate that ex vivo–expanded EPCs as well as murine hematopoietic Sca-1+/Lin progenitor cells express β2-integrins, which mediate the adhesion of EPCs to endothelial cell monolayers and their chemokine-induced transendothelial migration in vitro. In a murine model of hind limb ischemia, Sca-1+/Lin hematopoietic progenitor cells from β2-integrin–deficient mice are less capable of homing to sites of ischemia and of improving neovascularization. Preactivation of the β2-integrins expressed on EPCs by activating antibodies augments the EPC-induced neovascularization in vivo. These results provide evidence for a novel function of β2-integrins in postnatal vasculogenesis.
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