Mesenchymal stem cells differentiate into tenocytes by bone morphogenetic protein (BMP) 12 gene transfer
QW Wang, ZL Chen, YJ Piao - Journal of bioscience and bioengineering, 2005 - Elsevier
Rhesus bone marrow mesenchymal stem cells (MSCs) were transfected with the BMP12
gene by electroporation, and the phenotype of the transfected cells was identified by
morphological observation and molecular biological assay. After transfection, cells became
slender, and their processes became thinner and were interwoven into a network. There
were more organelles in the transfected cells than in the parental MSCs. The transfected
cells exhibited mRNA expressions of BMP12, collagen type I and scleraxis, but not collagen …
gene by electroporation, and the phenotype of the transfected cells was identified by
morphological observation and molecular biological assay. After transfection, cells became
slender, and their processes became thinner and were interwoven into a network. There
were more organelles in the transfected cells than in the parental MSCs. The transfected
cells exhibited mRNA expressions of BMP12, collagen type I and scleraxis, but not collagen …