Eosinophil chemotactic chemokines (eotaxin, eotaxin-2, RANTES, monocyte chemoattractant protein-3 (MCP-3), and MCP-4), and CC chemokine receptor 3 …

S Ying, Q Meng, K Zeibecoglou… - The Journal of …, 1999 - journals.aai.org
S Ying, Q Meng, K Zeibecoglou, DS Robinson, A Macfarlane, M Humbert, AB Kay
The Journal of Immunology, 1999journals.aai.org
Atopic (AA) and nonatopic (NAA) asthma are characterized by chronic inflammation and
local tissue eosinophilia. Many CC chemokines are potent eosinophil chemoattractants and
act predominantly via the CCR3. We examined the expression of eotaxin, eotaxin-2,
RANTES, monocyte chemoattractant protein-3 (MCP-3), MCP-4, and CCR3 in the bronchial
mucosa from atopic (AA) and nonatopic (intrinsic; NAA) asthmatics and compared our
findings with atopic (AC) and nonatopic nonasthmatic controls (NC). Cryostat sections were …
Abstract
Atopic (AA) and nonatopic (NAA) asthma are characterized by chronic inflammation and local tissue eosinophilia. Many CC chemokines are potent eosinophil chemoattractants and act predominantly via the CCR3. We examined the expression of eotaxin, eotaxin-2, RANTES, monocyte chemoattractant protein-3 (MCP-3), MCP-4, and CCR3 in the bronchial mucosa from atopic (AA) and nonatopic (intrinsic; NAA) asthmatics and compared our findings with atopic (AC) and nonatopic nonasthmatic controls (NC). Cryostat sections were processed for immunohistochemistry (IHC), in situ hybridization (ISH), and double IHC/ISH. Compared with AC and NC, the numbers of EG2+ cells and the cells expressing mRNA for eotaxin, eotaxin-2, RANTES, MCP-3, MCP-4, and CCR3 were significantly increased in AA and NAA (p< 0.01). Nonsignificant differences in these variants were observed between AA and NAA and between AC and NC. Significant correlations between the cells expressing eotaxin or CCR3 and EG2+ eosinophils in the bronchial tissue were also observed for both AA (p< 0.01) and NAA (p= 0.01). Moreover, in the total asthmatic group (AA+ NAA) there was a significant inverse correlation between the expression of eotaxin and that of the histamine PC 20 (p< 0.05). Sequential IHC/ISH showed that cytokeratin+ epithelial cells, CD31+ endothelial cells, and CD68+ macrophages were the major sources of eotaxin, eotaxin-2, RANTES, MCP-3, and MCP-4. There was no significantly different distribution of cells expressing mRNA for these chemokines between atopic and nonatopic asthma. These findings suggest that multiple CC chemokines, acting at least in part via CCR3, contribute to bronchial eosinophilia in both atopic and nonatopic asthma.
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