[HTML][HTML] Cleavage of the matricellular protein SPARC by matrix metalloproteinase 3 produces polypeptides that influence angiogenesis

EH Sage, M Reed, SE Funk, T Truong… - Journal of Biological …, 2003 - ASBMB
SPARC, a matricellular protein that affects cellular adhesion and proliferation, is produced in
remodeling tissue and in pathologies involving fibrosis and angiogenesis. In this study we
have asked whether peptides generated from cleavage of SPARC in the extracellular milieu
can regulate angiogenesis. Matrix metalloproteinase (MMP)-3, but not MMP-1 or 9, showed
significant activity toward SPARC. Limited digestion of recombinant human (rhu) SPARC
with purified catalytic domain of rhuMMP-3 produced three major fragments, which were …