The retina in most mammals contains two types of macroglial cells—Müller cells, which span the entire thickness of the retina, and astrocytes, which are mainly confined to the nerve fibre layer^1–6. Whereas Müller cells are diffusely distributed in all vertebrate retinae, the presence and distribution of retinal astrocytes correlate with the presence and distribution of retinal blood vessels: retinae that are avascular contain no astrocytes^7,8; those that are diffusely vascularized contain diffusely distributed astrocytes^5–12; and those that are vascularized in a restricted region contain astrocytes only in the vascularized region^7,8,13. This striking correlation between vascularization and the presence of astrocytes led Stone and Dreher to postulate that retinal astrocytes are immigrants that enter the retina with its vasculature⁷, although others have suggested that they derive from Miiller cells¹⁴. Here we provide strong evidence that astrocytes in the diffusely vascularized rat retina are immigrants from the optic nerve.