[HTML][HTML] Liver-specific enhancer of the glucokinase gene
PB Iynedjian, S Marie, H Wang, A Gjinovci… - Journal of Biological …, 1996 - ASBMB
Glucokinase gene regions that are important for liver specific expression of the enzyme have
been functionally identified using transient transfection of rat hepatocytes. Maximal
luciferase activity was elicited by a reporter plasmid with 3.4 kilobase pairs of genomic DNA
flanking the liver glucokinase promoter. Deletion of a gene fragment between− 1000 and−
600 with respect to the start of transcription resulted in a 60% decrease in luciferase activity.
Further reduction, close to background level, occurred upon deletion of a 90-base pair …
been functionally identified using transient transfection of rat hepatocytes. Maximal
luciferase activity was elicited by a reporter plasmid with 3.4 kilobase pairs of genomic DNA
flanking the liver glucokinase promoter. Deletion of a gene fragment between− 1000 and−
600 with respect to the start of transcription resulted in a 60% decrease in luciferase activity.
Further reduction, close to background level, occurred upon deletion of a 90-base pair …