Human tissue factor contains thioester-linked palmitate and stearate on the cytoplasmic half-cystine
R Bach, WH Konigsberg, Y Nemerson - Biochemistry, 1988 - ACS Publications
R Bach, WH Konigsberg, Y Nemerson
Biochemistry, 1988•ACS PublicationsMaterials and Methods TF Isolation and Iodoacetamide Labeling. Human brain TF was
purified by immunoaffinity chromatography as pre-viously described (Carson et al., 1987;
Spicer et al., 1987). The isolated protein,=* 100 jug/mL in 0.1 M NaCl and 0.05 M Tris, pH
7.5 (TBS), containing 0.1% Triton X-100, was precipitated by addition of trichloroacetic acid
to give 10%
purified by immunoaffinity chromatography as pre-viously described (Carson et al., 1987;
Spicer et al., 1987). The isolated protein,=* 100 jug/mL in 0.1 M NaCl and 0.05 M Tris, pH
7.5 (TBS), containing 0.1% Triton X-100, was precipitated by addition of trichloroacetic acid
to give 10%
Materials and Methods
TF Isolation and Iodoacetamide Labeling. Human brain TF was purified by immunoaffinity chromatography as pre-viously described (Carson et al., 1987; Spicer et al., 1987). The isolated protein,=* 100 jug/mL in 0.1 M NaCl and 0.05 M Tris, pH 7.5 (TBS), containing 0.1% Triton X-100, was precipitated by addition of trichloroacetic acid to give 10%
ACS Publications