[HTML][HTML] Direct measurement of antigen binding properties of CD1 proteins using fluorescent lipid probes
JS Im, OA Karl, PA Illarionov, KP LeClair… - Journal of Biological …, 2004 - ASBMB
CD1 proteins are antigen-presenting molecules that bind foreign and self-lipids and
stimulate specific T cell responses. In the current study, we investigated ligand binding by
CD1 proteins by developing a fluorescent probe binding approach using soluble
recombinant human CD1 proteins. To increase stability and yield, soluble group 1 CD1
(CD1b and CD1c) and group 2 CD1 (CD1d) proteins were produced as single chain
secreted CD1 proteins in which β 2-microglobulin was fused to the N termini of the CD1 …
stimulate specific T cell responses. In the current study, we investigated ligand binding by
CD1 proteins by developing a fluorescent probe binding approach using soluble
recombinant human CD1 proteins. To increase stability and yield, soluble group 1 CD1
(CD1b and CD1c) and group 2 CD1 (CD1d) proteins were produced as single chain
secreted CD1 proteins in which β 2-microglobulin was fused to the N termini of the CD1 …