11-color, 13-parameter flow cytometry: identification of human naive T cells by phenotype, function, and T-cell receptor diversity
SC De Rosa, LA Herzenberg, LA Herzenberg… - Nature medicine, 2001 - nature.com
SC De Rosa, LA Herzenberg, LA Herzenberg, M Roederer
Nature medicine, 2001•nature.com1a CD45RA CD3 CD28 CD49f CD11a CD27 CD4 CD62L CD45RO CD8 CD57 2b CD45RA
CD11a CD28 EMA CD27 CD4 CD62L IFN-γ CD3 CD8 3c CD45RA CD3 Vβ Vβ CD11a
CD27 CD4 CD62L CD28 CD8 CD57 aStaining combination used for Figure 1. This and
similar combinations were used to generate the data in Fig. 2. bStaining combination used
for Fig. 3. A cascade yellow reagent was not included. EMA, ethidium monoazide bromide,
labels dead cells by intercalating into DNA and covalently binding to DNA when exposed to …
CD11a CD28 EMA CD27 CD4 CD62L IFN-γ CD3 CD8 3c CD45RA CD3 Vβ Vβ CD11a
CD27 CD4 CD62L CD28 CD8 CD57 aStaining combination used for Figure 1. This and
similar combinations were used to generate the data in Fig. 2. bStaining combination used
for Fig. 3. A cascade yellow reagent was not included. EMA, ethidium monoazide bromide,
labels dead cells by intercalating into DNA and covalently binding to DNA when exposed to …
1a CD45RA CD3 CD28 CD49f CD11a CD27 CD4 CD62L CD45RO CD8 CD57 2b CD45RA CD11a CD28 EMA CD27 CD4 CD62L IFN-γ CD3 CD8 3c CD45RA CD3 Vβ Vβ CD11a CD27 CD4 CD62L CD28 CD8 CD57 aStaining combination used for Figure 1. This and similar combinations were used to generate the data in Fig. 2. bStaining combination used for Fig. 3. A cascade yellow reagent was not included. EMA, ethidium monoazide bromide, labels dead cells by intercalating into DNA and covalently binding to DNA when exposed to light. cStaining combination used for Fig. 4. For each blood sample, PBMC were stained with all these reagents except for FITC and PE. The stained cells were divided in aliquots and then individually stained with seven different FITC/PE Vβ reagent combinations (see Methods).
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