Isolation and transplantation of allogeneic pulmonary endothelium derived from GFP transgenic mice
P Ewing, A Wilke, G Brockhoff, R Andreesen… - Journal of …, 2003 - Elsevier
The isolation of primary endothelial cells from murine tissues has long been a challenge and
remains a difficult task. Using GFP transgenic C57/BL6 mice as donors, we describe a
reliable method to isolate pulmonary endothelial cells by flow cytometry after staining with
DiI-Ac-low density lipoprotein (LDL). After mechanical dissociation of murine lung tissue and
enzymatic digestion, adherent cells can be quickly stained and sorted by flow cytometry. The
isolated cells express endothelial cell markers such as CD31, MECA32 and CD106 and …
remains a difficult task. Using GFP transgenic C57/BL6 mice as donors, we describe a
reliable method to isolate pulmonary endothelial cells by flow cytometry after staining with
DiI-Ac-low density lipoprotein (LDL). After mechanical dissociation of murine lung tissue and
enzymatic digestion, adherent cells can be quickly stained and sorted by flow cytometry. The
isolated cells express endothelial cell markers such as CD31, MECA32 and CD106 and …