[HTML][HTML] Hypochlorous acid oxygenates the cysteine switch domain of pro-matrilysin (MMP-7): a mechanism for matrix metalloproteinase activation and atherosclerotic …

X Fu, SY Kassim, WC Parks, JW Heinecke - Journal of Biological Chemistry, 2001 - ASBMB
Myeloperoxidase uses hydrogen peroxide (H 2 O 2) to generate hypochlorous acid (HOCl),
a potent cytotoxic oxidant. We demonstrate that HOCl regulates the activity of matrix
metalloproteinase-7 (MMP-7, matrilysin) in vitro, suggesting that this oxidant activates MMPs
in the artery wall. Indeed, both MMP-7 and myeloperoxidase were colocalized to lipid-laden
macrophages in human atherosclerotic lesions. A highly conserved domain called the
cysteine switch has been proposed to regulate MMP activity. When we exposed a synthetic …