Ura-status-dependent adhesion of Candida albicans mutants
JM Bain, C Stubberfield, NAR Gow - FEMS microbiology letters, 2001 - academic.oup.com
JM Bain, C Stubberfield, NAR Gow
FEMS microbiology letters, 2001•academic.oup.comGene disruptions in the diploid opportunistic human fungal pathogen Candida albicans are
usually created using multiple rounds of targeted integration called the 'ura-blaster'method.
Resulting heterozygous and homozygous null mutants can be auxotrophic (Ura−) or
prototrophic (Ura+) for uracil biosynthesis. Here we demonstrate that the Ura-status of
otherwise isogenic mutants affected the adhesion of C. albicans. Moreover the effect of Ura-
status on adhesion was also dependent on the null mutant background, the nature of the …
usually created using multiple rounds of targeted integration called the 'ura-blaster'method.
Resulting heterozygous and homozygous null mutants can be auxotrophic (Ura−) or
prototrophic (Ura+) for uracil biosynthesis. Here we demonstrate that the Ura-status of
otherwise isogenic mutants affected the adhesion of C. albicans. Moreover the effect of Ura-
status on adhesion was also dependent on the null mutant background, the nature of the …
Abstract
Gene disruptions in the diploid opportunistic human fungal pathogen Candida albicans are usually created using multiple rounds of targeted integration called the ‘ura-blaster’ method. Resulting heterozygous and homozygous null mutants can be auxotrophic (Ura−) or prototrophic (Ura+) for uracil biosynthesis. Here we demonstrate that the Ura-status of otherwise isogenic mutants affected the adhesion of C. albicans. Moreover the effect of Ura-status on adhesion was also dependent on the null mutant background, the nature of the underlying surface and the carbon source for growth. Therefore the Ura-status is not neutral in determining adhesive properties of C. albicans mutants that are generated via the ura-blaster protocol.
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