IL‐22 regulates the expression of genes responsible for antimicrobial defense, cellular differentiation, and mobility in keratinocytes: a potential role in psoriasis

K Wolk, E Witte, E Wallace, WD Döcke… - European journal of …, 2006 - Wiley Online Library
K Wolk, E Witte, E Wallace, WD Döcke, S Kunz, K Asadullah, HD Volk, W Sterry, R Sabat
European journal of immunology, 2006Wiley Online Library
Abstract IL‐22 is an IFN–IL‐10 cytokine family member, which is produced by activated Th1
and NK cells and acts primarily on epithelial cells. Here we demonstrate that IL‐22, in
contrast to its relative IFN‐γ, regulates the expression of only a few genes in keratinocytes.
This is due to varied signal transduction. Gene expressions regulated by IL‐22 should
enhance antimicrobial defense [psoriasin (S100A7), calgranulin A (S100A8), calgranulin B
(S100A9)], inhibit cellular differentiation (eg, profilaggrin, keratins 1 and 10, kallikrein 7), and …
Abstract
IL‐22 is an IFN–IL‐10 cytokine family member, which is produced by activated Th1 and NK cells and acts primarily on epithelial cells. Here we demonstrate that IL‐22, in contrast to its relative IFN‐γ, regulates the expression of only a few genes in keratinocytes. This is due to varied signal transduction. Gene expressions regulated by IL‐22 should enhance antimicrobial defense [psoriasin (S100A7), calgranulin A (S100A8), calgranulin B (S100A9)], inhibit cellular differentiation (e.g., profilaggrin, keratins 1 and 10, kallikrein 7), and increase cellular mobility [e.g., matrix metalloproteinease 1 (MMP1, collagenase 1), MMP3 (stromelysin 1), desmocollin 1]. In contrast, IFN‐γ favored the expression of MHC pathway molecules, adhesion molecules, cytokines, chemokines, and their receptors. The IL‐22 effects were transcriptional and either independent of protein synthesis and secretion, or mediated by a secreted protein. Inflammatory conditions, but not keratinocyte differentiation, amplified the IL‐22 effects. IL‐22 application in mice enhanced cutaneous S100A9 and MMP1 expression. High IL‐22 levels in psoriatic skin were associated with strongly up‐regulated cutaneous S100A7, S100A8, S100A9, and MMP1 expression. Psoriatic patients showed strongly elevated IL‐22 plasma levels, which correlated with the disease severity. Expression of IL‐22 and IL‐22‐regulated genes was reduced by anti‐psoriatic therapy. In summary, despite similarities, IFN‐γ primarily amplifies inflammation, while IL‐22 may be important in the innate immunity and reorganization of epithelia.
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