[PDF][PDF] Analysis of type 2 immunity in vivo with a bicistronic IL-4 reporter

M Mohrs, K Shinkai, K Mohrs, RM Locksley - Immunity, 2001 - cell.com
M Mohrs, K Shinkai, K Mohrs, RM Locksley
Immunity, 2001cell.com
Effector T cells mediate adaptive immunity and immunopathology, but methods for tracking
such cells in vivo are limited. We engineered knockin mice expressing IL-4 linked via a viral
IRES element with enhanced green fluorescent protein (EGFP). Reporter T cells primed
under Th2 conditions showed sensitive and faithful EGFP expression and maintained
endogenous IL-4. After Nippostrongylus infection, reporter expression demonstrated the
evolution of type 2 immunity from tissue lymphocytes and thence to lymph node CD4+ T …
Abstract
Effector T cells mediate adaptive immunity and immunopathology, but methods for tracking such cells in vivo are limited. We engineered knockin mice expressing IL-4 linked via a viral IRES element with enhanced green fluorescent protein (EGFP). Reporter T cells primed under Th2 conditions showed sensitive and faithful EGFP expression and maintained endogenous IL-4. After Nippostrongylus infection, reporter expression demonstrated the evolution of type 2 immunity from tissue lymphocytes and thence to lymph node CD4+ T cells, which subsequently migrated into tissue. The appearance of EGFP+ CD4+ T cells in tissue, but not in lymph nodes, was Stat6-dependent. Transferred EGFP+ CD4+ T cells from infected animals conferred protection against Nippostrongylus to immunodeficient mice. These mice will provide a valuable reagent for assessing immunity in vivo.
cell.com