Ursodeoxycholic acid induced activation of the glucocorticoid receptor in primary rat hepatocytes
C Weitzel, D Stark, F Kullmann… - European journal of …, 2005 - journals.lww.com
Methods Primary rat hepatocytes in culture were co-transfected with a luciferase reporter
gene construct (GRE-luc) containing a glucocorticoid receptor responsive element (GRE)
and a glucocorticoid receptor expression vector (6RGR) followed by stimulation with
dexamethasone or UDCA. Luciferase activity was determined and specific binding of
glucocorticoid receptor to the GRE was confirmed by an electrophoretic mobility shift assay
(EMSA). The glucocorticoid receptor binding site was expressed in a GR-myc fusion protein …
gene construct (GRE-luc) containing a glucocorticoid receptor responsive element (GRE)
and a glucocorticoid receptor expression vector (6RGR) followed by stimulation with
dexamethasone or UDCA. Luciferase activity was determined and specific binding of
glucocorticoid receptor to the GRE was confirmed by an electrophoretic mobility shift assay
(EMSA). The glucocorticoid receptor binding site was expressed in a GR-myc fusion protein …