[HTML][HTML] 3′-Untranslated region of phosphoenolpyruvate carboxykinase mRNA contains multiple instability elements that bind AUF1

S Hajarnis, JM Schroeder, NP Curthoys - Journal of Biological Chemistry, 2005 - ASBMB
Phosphoenolpyruvate carboxykinase (PEPCK) is regulated solely by alterations in gene
expression that involve changes in rates of PEPCK mRNA transcription and degradation. A
tetracycline-responsive promoter system was used to quantify the half-life of various
chimeric β-globin-PEPCK (βG-PCK) mRNAs in LLC-PK-F+ cells. The control βG mRNA was
extremely stable (t ½= 5 days). However, βG-PCK-1 mRNA, which contains the entire 3′-
UTR of the PEPCK mRNA, was degraded with a half-life of 1.2 h. RNase H treatment …