[HTML][HTML] Copper-catalyzed protein oxidation and its modulation by carbon dioxide: enhancement of protein radicals in cells
DC Ramirez, SEG Mejiba, RP Mason - Journal of Biological Chemistry, 2005 - ASBMB
It is well known that hydrogen peroxide (H 2 O 2)-induced copper-catalyzed fragmentation of
proteins follows a site-specific oxidative mechanism mediated by hydroxyl radical-like
species (ie Cu (I) O, Cu (II)/. OH or Cu (III)) that ends in increased carbonyl formation and
protein fragmentation. We have found that the nitrone spin trap DMPO (5, 5-dimethyl-1-
pyrroline N-oxide) prevented such processes by trapping human serum albumin (HSA)-
centered radicals, in situ and in real time, before they reacted with oxygen. When (bi) …
proteins follows a site-specific oxidative mechanism mediated by hydroxyl radical-like
species (ie Cu (I) O, Cu (II)/. OH or Cu (III)) that ends in increased carbonyl formation and
protein fragmentation. We have found that the nitrone spin trap DMPO (5, 5-dimethyl-1-
pyrroline N-oxide) prevented such processes by trapping human serum albumin (HSA)-
centered radicals, in situ and in real time, before they reacted with oxygen. When (bi) …
