Identification and characterization of 5′ extension of mammalian agrin cDNA, the exons and the promoter sequences
K Shigemoto, S Kubo, N Maruyama, S Yamada… - … et Biophysica Acta (BBA …, 2000 - Elsevier
Agrin, which is secreted from motor neurons, is essential for the formation and maintenance
of the vertebrate neuromuscular junctions. Here we show the complete N-terminal sequence
of the mammalian cDNA required for the expression and secretion as well as the intron/exon
structure and the 5′-flanking sequence required for basal promoter activity. The 5′-
flanking region and the first exon are extremely GC rich and contain a CpG island. These
features may account for hindrance in identification of the 5′ end of the cDNA and the …
of the vertebrate neuromuscular junctions. Here we show the complete N-terminal sequence
of the mammalian cDNA required for the expression and secretion as well as the intron/exon
structure and the 5′-flanking sequence required for basal promoter activity. The 5′-
flanking region and the first exon are extremely GC rich and contain a CpG island. These
features may account for hindrance in identification of the 5′ end of the cDNA and the …