[HTML][HTML] Alzheimer disease-specific conformation of hyperphosphorylated paired helical filament-Tau is polyubiquitinated through Lys-48, Lys-11, and Lys-6 ubiquitin …
One of the key pathological hallmarks of Alzheimer disease (AD) is the accumulation of
paired helical filaments (PHFs) of hyperphosphorylated microtubule-associated protein Tau.
Tandem mass spectrometry was employed to examine PHF-Tau post-translational
modifications, in particular protein phosphorylation and ubiquitination, to shed light on their
role in the early stages of Alzheimer disease. PHF-Tau from Alzheimer disease brain was
affinity-purified by MC1 monoclonal antibody to isolate a soluble fraction of PHF-Tau in a …
paired helical filaments (PHFs) of hyperphosphorylated microtubule-associated protein Tau.
Tandem mass spectrometry was employed to examine PHF-Tau post-translational
modifications, in particular protein phosphorylation and ubiquitination, to shed light on their
role in the early stages of Alzheimer disease. PHF-Tau from Alzheimer disease brain was
affinity-purified by MC1 monoclonal antibody to isolate a soluble fraction of PHF-Tau in a …