[HTML][HTML] A regulatory mechanism that detects premature nonsense codons in T-cell receptor transcripts in vivo is reversed by protein synthesis inhibitors in vitro
MS Carter, J Doskow, P Morris, S Li, RP Nhim… - Journal of Biological …, 1995 - ASBMB
Gene rearrangement during the ontogeny of T-and B-cells generates an enormous
repertoire of T-cell receptor (TCR) and immunoglobulin (Ig) genes. Because of the error-
prone nature of this rearrangement process, two-thirds of rearranged TCR and Ig genes are
expected to be out-of-frame and thus contain premature terminations codons (ptcs). We
performed sequence analysis of reverse transcriptase-polymerase chain reaction products
from fetal and adult thymus and found that newly transcribed TCR-β pre-mRNAs (intron …
repertoire of T-cell receptor (TCR) and immunoglobulin (Ig) genes. Because of the error-
prone nature of this rearrangement process, two-thirds of rearranged TCR and Ig genes are
expected to be out-of-frame and thus contain premature terminations codons (ptcs). We
performed sequence analysis of reverse transcriptase-polymerase chain reaction products
from fetal and adult thymus and found that newly transcribed TCR-β pre-mRNAs (intron …