Cytokine regulation of the myeloid glycoprotein CD14
R Landmann, M Wesp, JP Obrecht - Pathobiology, 1991 - karger.com
R Landmann, M Wesp, JP Obrecht
Pathobiology, 1991•karger.comAbstract Monocyte membrane CD 14 (mCD14) antigen expression was measured in normal
human monocytes and blood monocyte-derived macrophages. Seven-day culture of
monocytes in serum-containing medium lead to an increase in mCD14. Addition of
interferon-γ (IFNγ) or interleukin-4 (IL· 4) to monocytes caused a dose-dependent reduction
in mCD14 within 3 or 45 h respectively. These effects were strong in monocytes, weak in
macrophages, and they were blocked by anti-IFNγ and anti-IL-4 antibodies, respectively …
human monocytes and blood monocyte-derived macrophages. Seven-day culture of
monocytes in serum-containing medium lead to an increase in mCD14. Addition of
interferon-γ (IFNγ) or interleukin-4 (IL· 4) to monocytes caused a dose-dependent reduction
in mCD14 within 3 or 45 h respectively. These effects were strong in monocytes, weak in
macrophages, and they were blocked by anti-IFNγ and anti-IL-4 antibodies, respectively …
Abstract
Monocyte membrane CD 14 (mCD14) antigen expression was measured in normal human monocytes and blood monocyte-derived macrophages. Seven-day culture of monocytes in serum-containing medium lead to an increase in mCD14. Addition of interferon-γ (IFNγ) or interleukin-4 (IL·4) to monocytes caused a dose-dependent reduction in mCD14 within 3 or 45 h respectively. These effects were strong in monocytes, weak in macrophages, and they were blocked by anti-IFNγ and anti-IL-4 antibodies, respectively. Interleukin-2 and interferon-α produced a decrease in mCD14 in mononuclear leukocyte cultures but not in purified monocytes. Their effect was abolished in the presence of anti-IFNγ. Other cytokines (TFNα, Illβ, IL-6, IL-3, IL·5, GM-CSF, TGFβ1) did not change mCD14. In conclusion, IFNγ and IL-4 are revealed to be the only cytokines which directly affect monocyte CD 14 expression.
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