[HTML][HTML] Orai1 and STIM reconstitute store-operated calcium channel function
J Soboloff, MA Spassova, XD Tang… - Journal of Biological …, 2006 - ASBMB
The two membrane proteins, STIM1 and Orai1, have each been shown to be essential for
the activation of store-operated channels (SOC). Yet, how these proteins functionally interact
is not known. Here, we reveal that STIM1 and Orai1 expressed together reconstitute
functional SOCs. Expressed alone, Orai1 strongly reduces store-operated Ca 2+ entry
(SOCE) in human embryonic kidney 293 cells and the Ca 2+ release-activated Ca 2+
current (I CRAC) in rat basophilic leukemia cells. However, expressed along with the store …
the activation of store-operated channels (SOC). Yet, how these proteins functionally interact
is not known. Here, we reveal that STIM1 and Orai1 expressed together reconstitute
functional SOCs. Expressed alone, Orai1 strongly reduces store-operated Ca 2+ entry
(SOCE) in human embryonic kidney 293 cells and the Ca 2+ release-activated Ca 2+
current (I CRAC) in rat basophilic leukemia cells. However, expressed along with the store …