A simple free fat cell bioassay for insulin
AJ Moody, MA Stan, J Gliemann - Hormone and Metabolic …, 1974 - thieme-connect.com
AJ Moody, MA Stan, J Gliemann
Hormone and Metabolic Research, 1974•thieme-connect.comA simple bioassay is described for insulin-like activity (ILA) which is based on the
incorporation of (3H) glucose into the lipids of isolated rat fat cells. Total lipids were
extracted by adding toluene-based scintillation fluid directly to the incubation flasks, and the
radioactivity in the lipids measured by liquid scintillation counting. Insulin dose-response
curves with (2-3H)-,(3-3H) and (6-3H) glucose were compared and those obtained with (2-
3H) glucose and (3-3H) glucose were found suitable for routine use.
incorporation of (3H) glucose into the lipids of isolated rat fat cells. Total lipids were
extracted by adding toluene-based scintillation fluid directly to the incubation flasks, and the
radioactivity in the lipids measured by liquid scintillation counting. Insulin dose-response
curves with (2-3H)-,(3-3H) and (6-3H) glucose were compared and those obtained with (2-
3H) glucose and (3-3H) glucose were found suitable for routine use.
Abstract
A simple bioassay is described for insulin-like activity (ILA) which is based on the incorporation of (3H) glucose into the lipids of isolated rat fat cells. Total lipids were extracted by adding toluene-based scintillation fluid directly to the incubation flasks, and the radioactivity in the lipids measured by liquid scintillation counting. Insulin dose-response curves with (2-3H)-,(3-3H) and (6-3H) glucose were compared and those obtained with (2-3H) glucose and (3-3H) glucose were found suitable for routine use.
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