An improved protocol for measuring cytotoxic T cell activity in anatomic compartments with low cell numbers
T Ostler, K Schamel, T Hussell, P Openshaw… - Journal of …, 2001 - Elsevier
The study of target cell lysis and cytokine production are valuable tools to characterize
antigen-specific T and NK cell function during virus infections. After localized infections in
compartments such as the lung or the brain, however, cell numbers isolated from these
organs are too low to perform standard assays with individual mice. Here, we report a few
simple modifications of the classical 51Cr release assay allowing reduction of the number of
required effector cells by a factor of 10 without loosing sensitivity or specificity. Using not …
antigen-specific T and NK cell function during virus infections. After localized infections in
compartments such as the lung or the brain, however, cell numbers isolated from these
organs are too low to perform standard assays with individual mice. Here, we report a few
simple modifications of the classical 51Cr release assay allowing reduction of the number of
required effector cells by a factor of 10 without loosing sensitivity or specificity. Using not …