[HTML][HTML] Substrate specificities of recombinant mannan-binding lectin-associated serine proteases-1 and-2
V Rossi, S Cseh, I Bally, NM Thielens… - Journal of Biological …, 2001 - ASBMB
Mannan-binding lectin (MBL)-associated serine proteases-1 and 2 (MASP-1 and MASP-2)
are homologous modular proteases that each interact with MBL, an oligomeric serum lectin
involved in innate immunity. To precisely determine their substrate specificity, human MASP-
1 and MASP-2, and fragments from their catalytic regions were expressed using a
baculovirus/insect cells system. Recombinant MASP-2 displayed a rather wide, C1s-like
esterolytic activity, and specifically cleaved complement proteins C2 and C4, with relative …
are homologous modular proteases that each interact with MBL, an oligomeric serum lectin
involved in innate immunity. To precisely determine their substrate specificity, human MASP-
1 and MASP-2, and fragments from their catalytic regions were expressed using a
baculovirus/insect cells system. Recombinant MASP-2 displayed a rather wide, C1s-like
esterolytic activity, and specifically cleaved complement proteins C2 and C4, with relative …