The use of HLA-A* 0201-transfected K562 as standard antigen-presenting cells for CD8+ T lymphocytes in IFN-γ ELISPOT assays
CM Britten, RG Meyer, T Kreer, I Drexler… - Journal of …, 2002 - Elsevier
ELISPOT assays are increasingly used for a direct detection and quantification of single
antigen-specific T cells in freshly isolated peripheral blood mononuclear cells (PBMC). They
are particularly attractive for the monitoring of specific T lymphocyte responses in clinical
trials assessing antigen-specific immunizations in patients with cancer or chronic viral
infections. However, one major limitation for the broad clinical implementation of ELISPOT
assays is the lack of an inexhaustible source of suitable HLA-matched antigen-presenting …
antigen-specific T cells in freshly isolated peripheral blood mononuclear cells (PBMC). They
are particularly attractive for the monitoring of specific T lymphocyte responses in clinical
trials assessing antigen-specific immunizations in patients with cancer or chronic viral
infections. However, one major limitation for the broad clinical implementation of ELISPOT
assays is the lack of an inexhaustible source of suitable HLA-matched antigen-presenting …