Mannose receptor contribution to Candida albicans phagocytosis by murine E-clone J774 macrophages

I Porcaro, M Vidal, S Jouvert, PD Stahl… - Journal of Leucocyte …, 2003 - academic.oup.com
I Porcaro, M Vidal, S Jouvert, PD Stahl, J Giaimis
Journal of Leucocyte Biology, 2003academic.oup.com
Mannoproteins, as the main constituents of the outer layer of yeast cell walls, are able to
interact with phagocytic cells in an opsonin-independent manner through the mannose
receptor (MR) and to induce yeast ingestion by the professional phagocytes. Moreover, the
MR also mediates endocytosis of soluble ligands through clathrin-coated pits. Here, we
studied some aspects of the interaction between the MR and Candida albicans using murine
E-clone macrophages and the consequences on MR trafficking. Using a pull-down assay …
Abstract
Mannoproteins, as the main constituents of the outer layer of yeast cell walls, are able to interact with phagocytic cells in an opsonin-independent manner through the mannose receptor (MR) and to induce yeast ingestion by the professional phagocytes. Moreover, the MR also mediates endocytosis of soluble ligands through clathrin-coated pits. Here, we studied some aspects of the interaction between the MR and Candida albicans using murine E-clone macrophages and the consequences on MR trafficking. Using a pull-down assay involving mixture E-clone macrophage detergent lysate with mannosylated Sepharose beads and glutaraldehyde-fixed, heat-killed (HK) C. albicans, we found that binding of solubilized MR to mannosylated particles occurred with characteristics similar to the receptor’s cell-surface mannose-binding activity. We then demonstrated that MR expressed on E-clone macrophages contributed to phagocytosis of unopsonized, HK C. albicans and that yeast phagocytosis induced a decrease in MR endocytic activity without concomitant degradation of the receptor in the time lapse studied.
Oxford University Press