[HTML][HTML] 13C NMR isotopomer analysis of anaplerotic pathways in INS-1 cells
GW Cline, RL LePine, KK Papas, RG Kibbey… - Journal of Biological …, 2004 - ASBMB
Anaplerotic flux into the Kreb's cycle is crucial for glucose-stimulated insulin secretion from
pancreatic β-cells. However, the regulation of flux through various anaplerotic pathways in
response to combinations of physiologically relevant substrates and its impact on glucose-
stimulated insulin secretion is unclear. Because different pathways of anaplerosis generate
distinct products, they may differentially modulate the insulin secretory response. To
examine this question, we applied 13 C-isotopomer analysis to quantify flux through three …
pancreatic β-cells. However, the regulation of flux through various anaplerotic pathways in
response to combinations of physiologically relevant substrates and its impact on glucose-
stimulated insulin secretion is unclear. Because different pathways of anaplerosis generate
distinct products, they may differentially modulate the insulin secretory response. To
examine this question, we applied 13 C-isotopomer analysis to quantify flux through three …