[HTML][HTML] Differential effects of GLUT1 or GLUT4 overexpression on hexosamine biosynthesis by muscles of transgenic mice
MG Buse, KA Robinson, BA Marshall… - Journal of Biological …, 1996 - ASBMB
Transgenic mice that overexpress GLUT1 or GLUT4 in skeletal muscle were studied; the
former but not the latter develop insulin resistance. Because increased glucose flux via the
hexosamine biosynthesis pathway has been implicated in glucose-induced insulin
resistance, we measured the activity of glutamine: fructose-6-phosphate amidotransferase
(GFAT; rate-limiting enzyme) and the concentrations of UDP-N-acetyl hexosamines (major
products of the pathway) as well as UDP-hexoses and GDP-mannose in hind limb muscles …
former but not the latter develop insulin resistance. Because increased glucose flux via the
hexosamine biosynthesis pathway has been implicated in glucose-induced insulin
resistance, we measured the activity of glutamine: fructose-6-phosphate amidotransferase
(GFAT; rate-limiting enzyme) and the concentrations of UDP-N-acetyl hexosamines (major
products of the pathway) as well as UDP-hexoses and GDP-mannose in hind limb muscles …